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FACS assessment of complex internalization to cells. Quantification of the internalization of EVs:PEI:Cy5-siRNA complexes prepared manually or including the device washing step, mean ± SD (n= 3), *p= 0.02 Device (EV)-PEI:siRNA vs EV-PEI:siRNA, ****p= 0.00003 Device (EV)-PEI:siRNA vs unmodified device, ***p= 0.0003 EV-PEI:siRNA vs unmodified device.
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