Microscopy from Scientific Research

Characterization of lumen formation on square grid scaffolds at day 2 and day 5 by staining for F-actin (phalloidin), and immunostaining with apical protein marker ZO1 and nuclei (DAPI). A representative example of lumen emergence at the scaffold intersections is shown on day 2 (white line arrowheads) and at the scaffold walls (solid white arrowheads). The bottom row shows the matured tissue comprising the lumen at day 5 (n= minimum of 3 tissue nodes from 1 or 2 independent scaffolds). Scale bar: 200 m.

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