Microscopy from Scientific Research

Representative tiled overview images of the entire scaffold (i) and magnified views (ii,iii) on day 2 and day 5 after hESC seeding. A representative example of lumen emergence is shown on day 2 (black arrowhead) and an established lumen on day 5 (white arrowhead). The red dotted lines with arrow bars denote the region of tissue thickness measurement for the fiber walls (T1) and intersection (T2) on the scaffolds. Scale bars: (ii) 500 µm, and (iii) 200 µm. (N= minimum of 2 independent scaffolds).

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