Observed DED map and superimposed dark (cyan) and light (yellow) models around the final tryptophan, Trp381, at 100 mus. The hydrogen bond between the backbone of Pro329 and Trp381 in the dark structure is shown as a dashed line (blue). In all maps, the negative and the positive features are depicted in gold and blue, respectively. Arrows indicate movement of atoms upon photoexcitation.