The integrated DED is displayed for the three regions as a function of delay time. Integration was over a radius of 1 A around the atoms of each region and for DED above 3.1 sigma of the map. The plot for each region is normalized at its maximum. The error bars indicate the noise floor of the DED map from which the data point was extracted, as estimated by the average of the integrated signal (>3.1 sigma ) over 5,000 randomly selected regions in the map, each consisting of six residues, excluding the residues in regions 1-3. The error bars are scaled with the same normalization factor as was used for the intensities for regions 1-3.
