Microscopy from Scientific Research

Left: representative phase-contrast/green fluorescence images of differentiated iNSLCs derived from TH-1 fibroblasts (Figure 3) on days 0, 2, and 7. For immunocytochemistry of TH, we used a TH-specific primary antibody and an Alexa 488-conjugated secondary antibody. Scale bars, 100 m. Right: western blotting of TH and -actin (internal control). Extracted protein solution (approximately 10 g/lane) from adult marmoset striatum differentiated TH-1 iNSLCs on days 0 and 7 was applied and immunodetected as described previously described.24.

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