Line Plot from Scientific Research

Open access visualization of Line Plot, Sanger sequencing, KI alleles, Cas9, sgRNA-1
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DOI: 10.1016/j.crmeth.2023.100590

DOI

Sanger sequencing of KI alleles (the 5.2-kb bands shown in Figure 1D) of Cas9+sgRNA-1 #1 (He KI) and Cas9+sgRNA-1 #2 (Ho KI) clones. Precise KI was confirmed by sequencing of the junction of the 5' side of P2A-Cre (shown above) and 3' side (data not shown).

#Line Plot#Sanger sequencing#KI alleles#Cas9#sgRNA-1#P2A-Cre

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