Line Plot from Scientific Research

PBMCs from healthy human donor (#7) treated with 1 μM of the PARP inhibitor olaparib (Olap) for 24 h prior to treatment with ara-C and ida for 24 h. Toxicity rescue was determined using a differential staining cytotoxicity assay, and nuclear fragmentation patterns were analyzed using DAPI staining, cytospin centrifugation, and confocal microscopy. Semi-quantitative image analyses of data from all 10 donor samples (Data S1) indicate that the ring-shaped nuclear patterns were observed in 14%–46% of the treated PBMCs as compared with only 0.3%–2.7% of the untreated PBMCs. Cells treated with either ara-C or ida alone also exhibited the characteristic DNA “ring” morphologies (Data S2).

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