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MDS-L-2007/Cas9 cells transduced with non-targeting (NT) or DNMT1-targeting sgRNAs (ko1 and ko2) were then transduced with vector or Dnmt1-WT. These cells were treated with DMSO or DAC (0.1 μM) over 72 h and subjected to FACS analysis to assess ploidy (left) and apoptosis (right).*p < 0.01,***p < 0.001.
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