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SPP1 concentration in aqueous humor was quantified using ELISA from patients with mild (n = 11) and severe (n = 13) forms of primary open-angle glaucoma (POAG) and age-matched controls (n = 16). Significance was assessed by one-way ANOVA followed byTukey's Honest Significant Difference (HSD) test for pairwise comparisons, demonstrating a significant difference between patients with severe glaucoma and control patients (p = 0.02). AH, aqueous humor.

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