Interaction matrices at 20-kb resolution for the Igh baited region for (A) pro-B and (B) thymus datasets. Each matrix entry is a coverage normalized interaction frequency value for the pairwise interaction between two 20-kb bins. Matrices from two biological replicates have been averaged to produce matrices (A) and (B). White lines are bins with too-low read coverage excluded from analysis by hypergeometric optimization of motif enrichment (HOMER). Arrow 1, 3' CBEs; arrow 2, Eμ; arrow 3, IGCR1; arrow 4, most proximal VH genes; arrow 5, 5' of 7183 VH gene family; arrow 6, 5' of S107 VH gene family; arrow 7, 3' J606 VH genes; arrow 8, start of distal subdomain; arrow 9, end of distal subdomain. The positions of subdomains in pro-B cells determined using HiCseg are indicated by gray rectangles. See also Figures S2 and S3 and Table S2.
