Workflow from Scientific Research

Capture Hi-C (CHi-C) workflow. Top left: cross-linked chromatin was used to generate Hi-C libraries. Top right: BAC DNA was digested, sonicated, and in vitro transcribed to yield a library of biotinylated RNA baits. The Hi-C and RNA bait libraries were hybridized together and baited fragments captured with streptavidin beads. Captured sequences were amplified and paired-end sequenced. The resulting CHi-C libraries yielded intralocus Igh reads (bottom left) and interchromosomal reads (bottom right).

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