Line Plot from Scientific Research

Michaelis-Menten kinetics reveal a 10x higherKm of ceTIR-1 relative to hSARM1. Similar enzyme concentrations (260 nM) were incubated with varying NAD+concentrations for 10 min (hSARM1) or 30 min (ceTIR-1). Inhibition by the NAD+substrate is observed in hSARM1 but not ceTIR-1. Data points represent the mean of three measurements. The kinetic parameters were determined from plots of reaction velocity of NAD+consumption versus substrate (NAD+) concentration and then fitted to the Michaelis-Menten equation (Km andVmax) or substrate inhibition equation (Ki) using non-linear curve fit in GraphPad Prism. Kcat was calculated by dividing theVmax with protein molar concentration.

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