Microscopy from Scientific Research

Representative cell line for N-terminally tagged IMM proteins displayed only partial mitochondrial delivery, as they likely become immobilized in the OMM. Schematics shows mode of proposed OMM immobilization of aforementioned N-terminally tagged proteins versus delivery to the IMM upon C-terminal tagging. We propose that the internal targeting peptide first docks the entire protein on the OMM receptor protein, and then the N-terminal targeting peptide facilitates the insertion into the membrane.

DOI