Microscopy from Scientific Research

Double ISH staining of pri-miR-2022 with its two targets in 4-day-old Nematostella planulae. Samples were sliced into 14 m sections to reduce background staining noise. In the two upper panels, DIG-labelled probes and NBT/BCIP staining were used for pri-miR-2022 staining, and FITC-labelled probes and FastRed staining were used for the targets. In the second from bottom panel, DIG-labelled probes and NBT/BCIP staining, and FITC-labelled probes with FastRed staining were used for visualization of nve16498 and nve16448, respectively, Scale bars, 100 m.

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