The RNA metric complements the DNA metric. Genes on the X axis (DNA metric) are ranked by their depletion of LoF (observed/expected, estimation lower bound). The left bin is solely composed with genes o/e upper bound <0.5 (DNA metric high confidence constrained), while the right bin is solely with those with o/e lower bound >0.5 (DNA metric high confidence unconstrained). The middle bin is composed of genes where the DNA metric is underpowered (o/e lower bound < 0.5 and upper bound > 0.5 or ambiguous confidence interval due to short gene length. The DNA metric confidence interval as affected by gene length is illustrated inFigure S12). The y axis is the RNA metric (aFC). For each gene, maximum aFC across tissues is plotted (median aFC across tissues is shown inFigure S11).
