Microscopy from Scientific Research

Time-lapse imaging of TMMp:OPL2-BFP; BRXL2p:BRXL2-YFP; ML1p:mCherry-RCI2A from 0 h (H) to 12 h (N). Boxed cell undergoing an ACD is followed through intermediate time points (I–M). OPL2-BFP (blue) polarizes opposite of BRXL2-YFP (yellow; K) and occupies the new cell plate during ACD (L and Lʹ) before the mCherry-RCI2A PM marker (L″). Scale bars, 20 μm in (H and N) or 10 μm (I–M). See also Figure S1 and Video S1.

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