Quantified RH length of Col-0, rhd6-4, two independent transgenic lines expressing either pro35S::LRL3::GFP or proEXP7::LRL3::GFP constructs in the rhd6-4 background (H) after 4 days of cultivation under HN or LN. n = 8 independent biological replicates grown under each respective N condition. Each symbol in (A) and (C) represents the value of one individual biological replicate. Red symbols with crossbars represent mean ± SEM. In (E), horizontal lines indicate medians, box limits the 25th and 75th percentiles, and whiskers extend to 1.5 times the interquartile range from the 25th and 75th percentiles. Different letters indicate significant differences at p < 0.001 according to one-way ANOVA and post hoc Tukey test. Scale bars, 100 μm.
