Quantified RH length of LRL3 overexpression plants after 4 days of cultivation under HN or LN. Number of individual roots analyzed in HN/LN: n = 10/10 (Col-0), 12/12 (lrl3-1), 8/8 (lrl3-2), 8/8 (lrl1lrl3), and 8/8 (lrl2lrl3) in (D) and n = 14/13 (Col-0) and 14/13 (LRL3OX) in (J). The p value was calculated by Welch’s t test. In (C), (E), and (F), each symbol represents the value of one individual biological replicate. Red symbols with crossbars represent mean ± SEM. p values were calculated by Welch’s t test. Horizontal lines in box plots indicate medians, box limits the 25th and 75th percentiles, and whiskers extend to 1.5 times the interquartile range from the 25th and 75th percentiles. Different letters indicate significant differences at p < 0.05 according to one-way ANOVA and post hoc Tukey test. Scale bars, 100 μm in (B), (D), and (L) and 500 μm in (G) and (I). See also Figure S4 and Data S3.
