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Choanoflagellate ciliogenesis can be synchronized and quantified following ciliary removal. To this end, S. rosetta cells were treated with 10% glycerol and cold-shocked (STAR Methods), which results in the severing of cilia. We observed that nascent cilia sometimes collapse and resorb before a new round of ciliary growth begins (gray arrows). The point at which the growing cilium passed the edge of the microvillar collar was used as a marker of successful ciliogenesis (asterisk).

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