Choanoflagellate cRFXa genes form a clade with the animal RFX1/2/3 sub-family. This maximum-likelihood phylogenetic tree includes RFX predicted protein sequences from diverse opisthokonts and amoebozoans (Data S1, File S1). Width of branches indicates scales with UFboot support for the ancestral node and all nodes with less than 75% bootstrap support are collapsed. Labels A, B, and C indicate ancestral nodes of homologous choanoflagellate/animal RFX sub-families. Node A has 81% bootstrap support, Node B has 81% bootstrap support, and Node C has 85% bootstrap support. Branch lengths do not scale with evolutionary distance in this rendering. See Figure S1B for full annotated version of this phylogeny, including branch lengths, bootstrap values, and all species names. See Figure S1C for phylogenetic trees built with different trimming and reconstruction algorithms. See also Data S1.
