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Parthenogenetically produced offspring from the induced facultative parthenogenetic D. melanogaster lines or produced naturally from the facultative parthenogenetic D. mercatorum were backcrossed to the parental line to determine if they could produce progeny in the F2 generation. GFP-polo+ is a transgene on the X, Desat2- is a 5 UTR deletion, Desat1- is a TE insertion, Myc dp is a gene duplication on the 3rd chromosome, and slmb- is a null allele. See also Figure S4 and Data S6B.

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