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Residue-specific changes in the HSQC peak intensities of Tau upon binding to full-length G3BP2, the G3BP2 NTF2-like domain, and the G3BP2 acidic + PxxP fragment. The red and blue bars indicate data collected with Tau:G3BP2 at molar ratios of 1:1 and 1:2, respectively. The green bars indicate data collected with Tau:G3BP2 NTF2-like at a molar ratio of 1:2. The orange bars indicate data collected with Tau:G3BP2 acidic + PxxP at a molar ratio of 1:2. The gray rectangle highlights the region for which the HSQC peak intensities significantly changed. The domain organization of Tau is shown above the graph.
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