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Western blot analysis in stones::3xhaline using an α-HA antibody under reducing conditions on Triton X-100 soluble (TriSol) and Triton X-100 insoluble (TriInsol) fractions. STONES::3xHA-specific signals indicated with black arrowheads. GFP and P28 used as loading and stage-specific controls, respectively; BS, mixed blood stages; Gc(-), inactivated purified in vitro cultured gametocytes; Gc(+), activated purified in vitro cultured gametocytes; Ook, purified in vitro cultured ookinetes.

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