(top) The average morphology of nucleoli ranked from lowest to highest sphericity and engineered droplets. Scale bars, 5 m. Representative images (top) and quantification of nucleolar morphology (bottom) for multiple cell types. Morphology is quantified by sphericity, where V and A publications are the volume and area, respectively; deviation from 1 indicates nonspherical shape. Error bars show cell-average standard error of the mean with smaller transparent points showing the individual volume-averaged nucleoli for each cell analyzed or each droplet analyzed for the case of HEK corelets. The number of cells (nucleoli) analyzed are 23 (68), 99 (803), 20 (81), 28 (42), and 13 (38) for IPSC, HEK, U2OS, MV, and rat neurons, respectively.
