Gel from Scientific Research

The mean level of intact Wis1 (band mobility ~100 kDa) ± SEM relative to Sty1WT(MC2) as determined from 3–9 biological repeats indicates that, although total Wis1 levels (SDS-extracted) are similar, the levels of intact Wis1 in extracts prepared under native conditions were ~3× higher in cells expressing Sty1-Tpx1 (MC12) (t test p = 0.0018) or Sty1-Tpx1C48S(MC82) (t test p = 0.0010) fusion proteins compared with wild-type Sty1 (MC2). Levels of Wis1 expression (SDS-extracted) may be lower in Sty15CS-Tpx1 (MC13) than in wild-type cells (t test = 0.064).

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