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Immunoblot analysis of cells expressing sty1WT (AD38), sty1-tpx1 (MG17), and sty1-tpx1C48S (MG18) before and following 10 min exposure to the indicated concentrations of H2O2. Antibodies against the dual TGY motif phosphorylated P38 (anti-ppP38) were used to detect phosphorylated Sty1 or Sty1-Tpx1 fusion proteins and total Sty1/Sty1-Tpx1 levels using anti-Hog1 antibodies. Total protein stain indicates protein loading, and molecular weight (MW) (kDa) marker positions are indicated. The relative levels of phosphorylated Sty1/total Sty1 are shown below each lane.

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