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Phosphorylation of P38 in HEK-293T cells ectopically expressing enhanced GFP (control), FLAG epitope-tagged P38, P38-Prdx1, P38-Prdx2, or P38-Prdx fusion proteins in which the indicated cysteines in Prdx1 or 2 are serine-substituted. The graph shows the mean relative phosphorylation of each protein compared with total protein (anti-FLAG) ± standard deviation for the two biological repeats shown. See also Figures S1 and S2.

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