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Immunoblotting analysis of the Sty1-stabilized substrate, Pyp2, in sty1WT (EB15)- or sty1-tpx1C48S (EB16)-expressing cells co-expressing myc-tagged Pyp2 from the Sty1-independent nmt1 promoter3 before and after 30 min exposure to 1.0 mM H2O2.
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