Line Plot from Scientific Research

(F′ and F″) Confocal images of live HeLa cells expressing a mitochondria-targeted BFP2 construct (Mito-sBFP2), EGFP-NEMO, and untagged Parkin over the course (min) of AntA/OligA treatment. In (F′), arrowheads mark a gradually forming NEMO puncta. In (F″), arrow marks a preexisting NEMO punctum near a mitochondrion. Arrowheads mark the subsequent interaction between the puncta and mitochondrion. Bottom, trace of average fluorescent intensity of NEMO recruited to mitochondria over time in 38 events following mitochondrial depolarization at 0 min. Data from 3 independent experiments. Error bars indicate SEM (seeVideo S1).

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