Violin plots of the buckling amplitude of the myoplasm before fertilization (unfertilized) and at 3, 6 and 13 mpf. ( N = 9 animals, n = 14 oocytes.) Solid lines, median. Dashed lines, quartiles. One-way analysis of variance and Holm–Sidak multiple comparisons test (* P = 0.0184, **** P < 0.0001).