Protein Structure from Scientific Research

Functional determinants of the TPO-MPL site 2 interface. (Top) TPO is shown as a pink ribbon, and MPL is shown as cyan transparent surface representation. The five TPO residues selected for the initial mutagenesis screening, including two tunable ones, K35TPOand R119TPO, subjected to further signaling and functional characterization, are shown as sticks. (Bottom) Close-up views of K35TPOand R119TPOinteracting with MPL. The cryo-EM map is shown as a gray transparent surface and overlaid on the structure at a contour level of 10sigma.

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