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Nucleosome remodeling assays with 0N60 mononucleosomes (147-bp Widom 601 DNA plus 60-bp linker) were performed with octamers of H2B, H4, and combinations of H3 and H2A variants as shown. Center-positioned nucleosomes (arrows) were incubated with DDM1, DDM1(C615S), or DDM1(Δ1-132) at t = 0 min, and then remodeled upon addition of ATP by putative sliding (slower migration) and unwrapping (faster migration) activities.

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