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qRT-PCR results (n = 4 biologically independent samples) of Atf4 -/- MEFs stably expressing vector, wild-type, 5A or F94A forms of ATF4, treated with or without Dox (30 μg/mL) for 24 h. Error bars denote SEM. Statistical analysis was performed by two-tailed unpaired Student’s t test in ( b ), or by ANOVA followed by Tukey post hoc test in ( a , c , e ) (** P < 0.01; *** P < 0.001; N.S. not significant).
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