Gel from Scientific Research

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Validation of the two commercially available antibodies that specifically recognize ATF4 phosphorylation at Ser 166 and Thr 173 , respectively. HEK293T cells transfected with the indicated plasmids were immunoprecipitated with anti-Flag antibody and analyzed by western blot assay. Torin1 (250 nM) was added 2 h before harvest.
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