Microscopy from Scientific Research

Immunofluorescent images of tight junction protein occludin (red) and vessel marker CD31 (green) in the BLA and ventral hippocampus of mice. Enlarged images of CD31-labeled vessels (square) showing downregulated occludin (arrowheads) in highly myopic mice. Scale bars: 40 m in white and 20 m in yellow. Fluorescence intensity normalized to the averaged value of the control for quantification analyses. Data are mean SD. n = 3 in each group ( a – c , e ); n = 6 in each group ( d ). Level of significance was detected with one-way repeated measures ANOVA ( a ) or Student’s t test ( b – e ). **** P < 0.0001, ***P < 0.001, **P < 0.01, * P < 0.05.

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