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Mean percentage delay of barcoded probes (not demultiplexed). Serum, after centrifugation with 10 kDa molecular weight cut-off spin filter, was incubated with 40 barcoded probes (30 nM) and compared to zero serum control (one-tailed t -test P > 0.05, n = 3, n total events = 194,115).

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