Scatter Plot from Scientific Research

Multiplexed detection of miR-208-5p, serotonin and thrombin. Low concentrations (10 nM miR-208-5p, 150 nM serotonin, 300 nM thrombin) and high concentration (50 nM miR-208-5p, 750 nM serotonin, 1,500 nM thrombin) were compared to the normalized control (no biomarker present). Normalized percentage delay of barcoded probes is shown (ANOVA, F (2,8) = [136.97], **** P = 6.48 x 10 -71 , F (2,8) = [4.74], * P = 4.38 x 10 -2 , F (2,8) = [28.57], *** P = 2.00 x 10 -4 , n = 3, n total events = 44,956). All data are presented as mean s.d. All experiments were performed in sequencing buffer. NA, not applicable.

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