Mean percentage delay 50 nM miRNAs (two-tailed t -test, **** P = 3.58 x 10 -4 , n = 5, n total events = 76,841 (0 nM), 69,983 (50 nM)). Data presented as mean s.d. (i). Multiplexed concentrationpercentage delay curves of 40 barcoded probes (30 nM) and the corresponding 40 miRNAs. A curve was fitted using the Hill equation with n H = 1 ( K d = 1.09 nM, V max = 38.69%, n = 5, n total events = 1,045,841). Data presented as mean s.d. (ii). Concentrationpercentage delay curves of 40 individual barcoded probes derived from a multiplexed experiment. Curves were fitted with the Hill fit ( n H = 1) background subtraction for each curve was performed using the percentage delay value at 0 nM for each curve ( n = 5, n total events = 1,045,841) (iii).
