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Metabolic activity of GIN 31 cells as a function of different-sized bio-nanoantennae (20 nm, 50 nm and 100 nm; GNP20@r.Cyt c @Z, GNP50@r.Cyt c @Z and GNP100@r.Cyt c @Z, respectively) synthesized using various linker lengths (1, 2, 3.5 and 5 kDa). GIN 31 cells were treated with bifunctionalized bio-nanoantennae for 8 h followed by a.c. EF stimulation (3 MHz, 0.65 V cm –1 ) for 12 h. Error bars represent ±s.e.m. of the mean obtained from triplicate experiments repeated three times. Statistical analysis was performed by applying a two-way ANOVA with a Tukey’s post-test. Exact P values are in Supplementary Table 7 .
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