Microscopy from Scientific Research

Open access visualization of Microscopy, Confocal Microscopy, Caspase 3/7 Activation, A.C. EFs, Bio-nanoantennae
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High-magnification confocal microscopy images to demonstrate caspase 3/7 activation immediately after the treatment with a.c. EFs (3 MHz, 0.65 V cm –1 ) for 12 h in the presence of bio-nanoantennae. Cells were fixed with paraformaldehyde followed by counterstaining with a caspase 3/7 detection kit (green), Cytopainter actin phalloidin (Texas Red 591, in red) and Hoechst nuclear stain (blue). Scale bars, 20 µm. To confirm caspase 3/7 activation, at least five confocal images (each with nearly 30 cells) were taken at ×10. High-resolution (×63) images show both morphology and caspase 3/7 activation (in green). The –EF and +EF indicate before and after the EF treatment.

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