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Quantification of FA length of MCF10A cells overexpressing WT β4 or mutant β4RW1218R integrin seeded on PAA gels coated with laminin-111 and on different rigidities (0.5–30.0 kPa) ( n = 40/33, 33/35, 31/35, 47/33, 27/19 and 36/36 cells for β4 and β4R1281W and increasing rigidity; mean of three independent experiments). The effect of rigidity is significant ( P < 0.0001, two-way ANOVA).
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