Protein Structure from Scientific Research

Comparison of weighted difference electron density maps ( F t – F dark2 ) for each pump–probe time delay, centred around residues 97–100. Maps were visualized at an absolute contour level of ±0.04 e − A − 3 alongside corresponding refined models. While model coordinates appeared stable across pump–probe time delays, difference maps revealed time-resolved changes to the T-jump induced signal, with evidence for coordinated motions (green arrows) apparent by 200 µs.

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