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FRAP of HERD-2.2–GFP (blue) and HERD-0–GFP (green) condensates in cells. The red circle indicates the bleached area. t 1/2 for HERD-2.2–GFP is 0.46 0.11 s. Data are represented as mean standard error from n = 13 (HERD-2.2–GFP) or n = 19 (HERD-0–GFP) biologically independent cells. Representative images of pre-bleach, post-bleach frame 1 and the final post-bleach frame shown alongside. Scale bar, 5 m. Common conditions for all experiments: 125 mM NaCl, 20 mM Tris–HCl, pH 7.5.
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