Microscopy from Scientific Research

Iteration of the HERD designs visualized by light microscopy. Fixed-cell confocal microscopy images of E. coli cells expressing (from top to bottom): soluble GFP (His-TEV-GFP; control), the initial HERD-0-GFP design, the final variant HERD-2.2-GFP, and a control construct with a monomeric helical region HERD-Ctrl2-GFP (control). GFP fluorescence images (488 nm) are shown left (green) and brightfield transmission images on the right (grey). Scale bars, 5 m.

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