Workflow from Scientific Research

Multiplexes are created by first generating de novo repeat protein monomers through backbone fragment assembly. A library of four-repeat monomers is generated, where a single repeat is consists of a two- or three-helix structural motif asymmetric. The monomer superhelical axis is calculated, and evenly spaced copies are generated around this axis. Different cyclic symmetries are attempted, and specific symmetries are selected according to contact number and clash score, and the designs are computationally filtered and experimentally characterized. In this panel only one backbone is selected for clarity, but in practice, all monomer backbones are run through the same design pipeline.

DOI