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Intracellular labelling efficiency of cSAMURI calculated from the Cy5/DFHBI-1T ratio, relative to the in vitro labelling efficiency of RNA extracted from cells that were not treated with ProSeDMA. The reference was prepared in the test tube using 200 ng total RNA, 50 M ProSeDMA and 10 mM MgCl 2 , pH 7.5 at 37 C for 1 h. Individual data points and the mean s.d. of three independent experiments are shown. RNA mod. rel. = relative intracellular RNA modification efficiency.
#Bar Plot#Error Bars#Intracellular Labeling Efficiency#cSAMURI#Cy5/DFHBI-1T Ratio#In Vitro Labeling Efficiency#ProSeDMA Treatment#RNA Modification
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