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LC/MS analysis of the digested 12-mer substrate RNA (5′-CUACUGAGCCUU-3′) before and after modification, showing the UV (260 nm) trace and extracted ion chromatogram (EIC, detecting m / z = 296.13). p 1 A = 1-propargyladenosine, p O2 A = 2′- O -propargyladenosine, p 6 A = N 6 -propargyladenosine.
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