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ChIP in HepG2 treated for 24 h with tamoxifen (10 M) or DMSO as a negative control. The levels of ER- at the ERE1, ERE2 and ERE3 of the PNPLA3 gene were measured by RT-qPCR. Data are presented as mean s.e.m. ( n = 3 independent experiments). The unpaired Student’s t -test was used.

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