Workflow from Scientific Research

Workflow for constructing Quartet RNA reference datasets. Reference datasets were constructed according to the following steps: (1) identifying detectable genes; (2) calculating ratio-based expression based on reliably detectable genes that were differentially expressed; (3) assessing the homogeneity and stability of RNA reference materials; (4) assessing the uncertainty of ratio-based reference datasets; and (5) identifying high-confidence DEGs.

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